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rna sequencing rna seq procedure  (Macrogen)

 
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    Macrogen rna sequencing rna seq procedure
    CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of <t>bulk</t> <t>RNA-seq</t> data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.
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    Images

    1) Product Images from "CD32b defines distinct dendritic cell lineages generated from the culture of bone marrow with GM-CSF"

    Article Title: CD32b defines distinct dendritic cell lineages generated from the culture of bone marrow with GM-CSF

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2026.1703978

    CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of bulk RNA-seq data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.
    Figure Legend Snippet: CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of bulk RNA-seq data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.

    Techniques Used: Derivative Assay, RNA Sequencing, Generated, Expressing, Biomarker Discovery, Marker, Fluorescence, Control

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    Article Snippet: .. Importantly, laboratory personnel remained unaware of the sample statuses throughout the process, and sequencing procedures were conducted at Macrogen after amplification. .. Gamma-irradiated H37Rv Mtb strain was acquired from Bei Resources, NIAID, NIH (Bethesda, MD, USA).

    Article Title: Phylogenetic Analysis and Genetic Diversity Among Some Red Sea Reef Fishes (Perciformes: Labridae) Based on Partial 16S Mitochondrial Gene Sequences
    Article Snippet: Sequencing reactions were performed in an MJ Research PTC-225 Peltier Thermal Cycler using an ABI PRISM® BigDyeTM Terminator Cycle Sequencing Kits with AmpliTaq® DNA polymerase (FS enzyme) (Applied Biosystems), following the manufacturer's protocols with the same primers used for PCR amplification. .. All sequencing procedures were carried out by Macrogen Inc., Seoul, Korea. .. Data analysis The newly generated 16S mt-DNA sequences were submitted to the GeneBank, National Center for Biotechnology Information (NCBI) (Table 1).

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    Article Snippet: Sequencing was performed on an Illumina MiSeq platform (MiSeq R2500) using 2×300 bp paired-end chemistry on multiplexed pooled samples. .. All sequencing procedures were conducted by Macrogen Inc. (Republic of Korea). ..

    Article Title: Enterotype-Specific Effects of Red Beetroot ( Beta vulgaris L.) Powder and Betanin on Human Gut Microbiota: A Preliminary Study Based on In Vitro Fecal Fermentation Model
    Article Snippet: After purifying the PCR products in the same manner, sequencing was performed using the Illumina MiSeq platform (Illumina, San Diego, CA, USA). .. All sequencing procedures were performed by Macrogen, Inc. (Seoul, Republic of Korea). ..

    Article Title: Precise Progerin Targeting Using RfxCas13d: A Therapeutic Avenue for Hutchinson-Gilford Progeria Syndrome.
    Article Snippet: Unbin Chae, Hae-Jun Yang, Hanseop Kim, Seung Hwan Lee, Dong Gil Lee, Jeong Young Koo, Seung-min Ha, Seo-Jong Bak, Mina Joo, Hyun Hee Nam, Kyung-Seob Lim, Philyong Kang, Hee-Chang Son, You Jeong An, Young-Hyun Kim, In-Sung Song, Sang-Hee Lee, Hae Rim Kim, Sang-Mi Cho, Eun-Kyoung Kim, Ki-Hoan Nam, Kyung-Sook Chung, Jae-Yoon Kim, Seon-Yeop Kim, Seon-Kyu Kim, Seon-Young Kim, Dong-Seok Lee, Jin-Man Kim, Young-Ho Park, Sun-Uk Kim

    Article Title: Complete Mitogenome and Phylogenetic Analysis of a Marine Ray-Finned Fish, Alcichthys elongatus (Perciformes: Cottidae)
    Article Snippet: Whole Genome Sequencing The Illumina Platform (Illumina Inc., San Diego, CA, USA) was used to sequence the genome of A. elongatus. .. The Macrogen Company (Daejeon, South Korea) took part in the library preparation and sequencing procedures. .. The TrueSeq® Nano DNA Kit was used to produce the DNA libraries in accordance with the manufacturer’s instructions, and the Illumina HiSeq 2500 (Illumina) was used for paired-end, 150 bp mode of sequencing.

    Article Title: Transition from Ginseng Root Rot Disease-Conducive Soil to -Suppressive Soil Mediated by Pseudomonadaceae .
    Article Snippet: .. The subsequent sequencing procedures were performed by Macrogen (Seoul, Republic of Korea). ..

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    Article Snippet: Sequencing was performed on an Illumina MiSeq platform (MiSeq R2500) using 2×300 bp paired-end chemistry on multiplexed pooled samples. .. All sequencing procedures were conducted by Macrogen Inc. (Republic of Korea). ..

    Amplification:

    Article Title: Variants of human DECTIN-1 rs16910526 are linked to differential reactive oxygen species production and susceptibility to tuberculosis
    Article Snippet: .. Importantly, laboratory personnel remained unaware of the sample statuses throughout the process, and sequencing procedures were conducted at Macrogen after amplification. .. Gamma-irradiated H37Rv Mtb strain was acquired from Bei Resources, NIAID, NIH (Bethesda, MD, USA).



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    Image Search Results


    USP18 Acts as a Key ROS-Responsive Mediator Driving Cisplatin Resistance in Ovarian Cancer. a Mechanisms and incidence of platinum resistance in ovarian cancer (Icons created with BioRender.com ). b Representative images of ROS detection using DCFH-DA probe in cisplatin-resistant and parental SKOV3 cells. Scale bar: 50 μm; GSEA analysis of ROS response genes based on transcriptome sequencing data in cisplatin-resistant and parental SKOV3 cells. c Representative images of ROS detection using DCFH-DA probe in SKOV3 cells treated with vehicle control, cisplatin (20 μM), or cisplatin plus NAC (10 μM) + mito-TEMPO (10 μM). Scale bar: 50 μm; Cell proliferation curves by Real-time cell analysis (RTCA) system (bottom left); LDH release assay in SKOV3 cells treated with cisplatin (20 μM) or cisplatin plus NAC (10 μM) + mito-TEMPO (10 μM) (bottom right). d Representative Immunohistochemistry (IHC) images showing pyroptosis (Cleaved-Caspase-1), apoptosis (Cleaved-Caspase-3), ferroptosis (GPX4), necroptosis (p-MLKL), and autophagy (p62) markers in cisplatin-resistant ovarian cancer PDX model (OV9419) tissues. Scale bar: 100 μm. e Protein expression levels of various cell death markers in OVCAR-3 cells treated with vehicle control, cisplatin (20 μM), or cisplatin plus NAC (10 μM) + mito-TEMPO (10 μM): pyroptosis (Cleaved-Caspase-1), apoptosis (Cleaved-Caspase-3), ferroptosis (GPX4), necroptosis (p-MLKL), and autophagy (LC3 II/LC3 I). f Schematic of DUB screening for cisplatin resistance: DUB plasmid library transfected into A2780 cells, treated with cisplatin (10 μM) after 24h, and cell viability measured by CCK8 after another 48h. Scatter plot shows relative cell viability compared to control. g Protein expression levels of USP18, USP37, and USP39 in parental and cisplatin-resistant SKOV3 cells. h Protein expression levels of USP18, USP37, and USP39 in OVCAR-3 cells treated with vehicle control, cisplatin (20 μM), or cisplatin (20 μM) plus NAC (10 μM) + mito-TEMPO (10 μM). i Protein expression levels of USP18, USP37, and USP39 in tissues from cisplatin-sensitive (n = 4) and -resistant (n = 4) ovarian cancer patients. j Analysis of USP18 expression in ovarian cancer versus normal ovarian tissues using data from The Cancer Genome Atlas (TCGA) ( www.aclbi.com ) k Survival analysis of patients with differential USP18 using data from TCGA. Analysis was performed via the KM-plotter platform ( https://kmplot.com/ ). l Representative IHC staining of USP18 shows its expression patterns in ovarian cancer tissues compared with paired adjacent tissues, and in therapy-sensitive versus resistant cases. Scale bar: 100 μm. Patients were stratified into high and low USP18 expression groups based on the median IHC score, and the distribution was compared using the Chi-squared test. m Analysis of USP18 expression in ovarian cancer patients stratified by response to platinum-based therapy using the ROC plot online platform ( https://rocplot.com/ ). n Protein expression levels of USP18 in ovarian cancer cell lines. o Cisplatin IC 50 values in ovarian cancer cell lines. p Pearson correlation analysis between USP18 protein levels (n) and cisplatin IC 50 (o). Data presented as mean ± SD. Significance calculated by unpaired two-tailed t -test (c). ∗∗P < 0.01; ∗∗∗∗P < 0.0001.

    Journal: Redox Biology

    Article Title: Redox-triggered USP18 confers cisplatin resistance in ovarian cancer by selectively activating a non-canonical FSP1-dependent ferroptosis escape pathway

    doi: 10.1016/j.redox.2026.104179

    Figure Lengend Snippet: USP18 Acts as a Key ROS-Responsive Mediator Driving Cisplatin Resistance in Ovarian Cancer. a Mechanisms and incidence of platinum resistance in ovarian cancer (Icons created with BioRender.com ). b Representative images of ROS detection using DCFH-DA probe in cisplatin-resistant and parental SKOV3 cells. Scale bar: 50 μm; GSEA analysis of ROS response genes based on transcriptome sequencing data in cisplatin-resistant and parental SKOV3 cells. c Representative images of ROS detection using DCFH-DA probe in SKOV3 cells treated with vehicle control, cisplatin (20 μM), or cisplatin plus NAC (10 μM) + mito-TEMPO (10 μM). Scale bar: 50 μm; Cell proliferation curves by Real-time cell analysis (RTCA) system (bottom left); LDH release assay in SKOV3 cells treated with cisplatin (20 μM) or cisplatin plus NAC (10 μM) + mito-TEMPO (10 μM) (bottom right). d Representative Immunohistochemistry (IHC) images showing pyroptosis (Cleaved-Caspase-1), apoptosis (Cleaved-Caspase-3), ferroptosis (GPX4), necroptosis (p-MLKL), and autophagy (p62) markers in cisplatin-resistant ovarian cancer PDX model (OV9419) tissues. Scale bar: 100 μm. e Protein expression levels of various cell death markers in OVCAR-3 cells treated with vehicle control, cisplatin (20 μM), or cisplatin plus NAC (10 μM) + mito-TEMPO (10 μM): pyroptosis (Cleaved-Caspase-1), apoptosis (Cleaved-Caspase-3), ferroptosis (GPX4), necroptosis (p-MLKL), and autophagy (LC3 II/LC3 I). f Schematic of DUB screening for cisplatin resistance: DUB plasmid library transfected into A2780 cells, treated with cisplatin (10 μM) after 24h, and cell viability measured by CCK8 after another 48h. Scatter plot shows relative cell viability compared to control. g Protein expression levels of USP18, USP37, and USP39 in parental and cisplatin-resistant SKOV3 cells. h Protein expression levels of USP18, USP37, and USP39 in OVCAR-3 cells treated with vehicle control, cisplatin (20 μM), or cisplatin (20 μM) plus NAC (10 μM) + mito-TEMPO (10 μM). i Protein expression levels of USP18, USP37, and USP39 in tissues from cisplatin-sensitive (n = 4) and -resistant (n = 4) ovarian cancer patients. j Analysis of USP18 expression in ovarian cancer versus normal ovarian tissues using data from The Cancer Genome Atlas (TCGA) ( www.aclbi.com ) k Survival analysis of patients with differential USP18 using data from TCGA. Analysis was performed via the KM-plotter platform ( https://kmplot.com/ ). l Representative IHC staining of USP18 shows its expression patterns in ovarian cancer tissues compared with paired adjacent tissues, and in therapy-sensitive versus resistant cases. Scale bar: 100 μm. Patients were stratified into high and low USP18 expression groups based on the median IHC score, and the distribution was compared using the Chi-squared test. m Analysis of USP18 expression in ovarian cancer patients stratified by response to platinum-based therapy using the ROC plot online platform ( https://rocplot.com/ ). n Protein expression levels of USP18 in ovarian cancer cell lines. o Cisplatin IC 50 values in ovarian cancer cell lines. p Pearson correlation analysis between USP18 protein levels (n) and cisplatin IC 50 (o). Data presented as mean ± SD. Significance calculated by unpaired two-tailed t -test (c). ∗∗P < 0.01; ∗∗∗∗P < 0.0001.

    Article Snippet: All sequencing procedures were performed by Annoroad Gene Tech. (Beijing) Co., Ltd.

    Techniques: Sequencing, Control, Cell Analysis, Lactate Dehydrogenase Assay, Immunohistochemistry, Expressing, Plasmid Preparation, Transfection, Two Tailed Test

    Elevated ROS levels upregulate c-JUN expression, which transcriptionally activates downstream USP18 in cisplatin-resistant ovarian cancer cells. a GSEA analysis of hallmark ROS pathway genes from transcriptome sequencing in OVCAR-3 cells overexpressing USP18 plasmid and vector. b Heatmap of transcriptome sequencing expression values for ROS pathway-related genes in A2780 cells overexpressing USP18 plasmid and vector. c Differentially expressed genes in OVCAR-3 cells treated with sodium arsenite (NaAsO2, 0.5 mM) versus vehicle control. d Differentially expressed genes in SKOV3 cells treated with cisplatin (20 μM) vs vehicle control. e Venn diagram of ROS pathway-related upregulated transcription factors identified from transcriptome sequencing data in c and d. f Analysis of JUN expression in ovarian cancer patients stratified by response to platinum-based therapy using the ROC plot online platform. g Survival analysis of patients with differential JUN expression using data from TCGA. Analysis was performed via the KM-plotter platform. h Protein expression levels of c-JUN and USP18 in OVCAR-3 cells treated with vehicle control, cisplatin (20 μM), or cisplatin (20 μM) plus NAC (10 μM) + mito-TEMPO (10 μM). i Protein expression levels of c-JUN and USP18 in OVCAR-3 cells treated with vehicle control, NaAsO2 (0.5 mM), or NaAsO2(0.5 mM) plus NAC (10 μM) + mito-TEMPO (10 μM). j Cell viability measured by CCK8 assay in OVCAR-3 and A2780 cells overexpressing USP18 and treated with cisplatin (10 μM) plus NAC (10 μM) + mito-TEMPO (10 μM) k RT-qPCR analysis of USP18 mRNA expression levels in OVCAR-3 cells treated with vehicle control, cisplatin (10 μM), or cisplatin combined with c-JUN knockdown. l Schematic of predicted c-JUN binding sites in the USP18 promoter region ( https://jaspar.elixir.no/ ). m ChIP assay in CAOV3 cells validating c-JUN binding to USP18. n-p Dual-luciferase reporter assay confirming c-JUN binding and transcriptional regulation of USP18. n Schematic diagram of the dual-luciferase reporter assay. o Overexpression of c-JUN activates the USP18 promoter. OVCAR-3/CAOV3 cells were co-transfected with the USP18 promoter reporter construct and a c-JUN expression plasmid (or empty vector control). p OVCAR-3 cells transfected with the USP18 promoter reporter were treated with vehicle control, cisplatin (10 μM), or cisplatin (10 μM) combined with c-JUN knockdown (sic-JUN). Data presented as mean ± SD. Significance calculated by unpaired two-tailed t -test (j, k, m, o, p). ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.

    Journal: Redox Biology

    Article Title: Redox-triggered USP18 confers cisplatin resistance in ovarian cancer by selectively activating a non-canonical FSP1-dependent ferroptosis escape pathway

    doi: 10.1016/j.redox.2026.104179

    Figure Lengend Snippet: Elevated ROS levels upregulate c-JUN expression, which transcriptionally activates downstream USP18 in cisplatin-resistant ovarian cancer cells. a GSEA analysis of hallmark ROS pathway genes from transcriptome sequencing in OVCAR-3 cells overexpressing USP18 plasmid and vector. b Heatmap of transcriptome sequencing expression values for ROS pathway-related genes in A2780 cells overexpressing USP18 plasmid and vector. c Differentially expressed genes in OVCAR-3 cells treated with sodium arsenite (NaAsO2, 0.5 mM) versus vehicle control. d Differentially expressed genes in SKOV3 cells treated with cisplatin (20 μM) vs vehicle control. e Venn diagram of ROS pathway-related upregulated transcription factors identified from transcriptome sequencing data in c and d. f Analysis of JUN expression in ovarian cancer patients stratified by response to platinum-based therapy using the ROC plot online platform. g Survival analysis of patients with differential JUN expression using data from TCGA. Analysis was performed via the KM-plotter platform. h Protein expression levels of c-JUN and USP18 in OVCAR-3 cells treated with vehicle control, cisplatin (20 μM), or cisplatin (20 μM) plus NAC (10 μM) + mito-TEMPO (10 μM). i Protein expression levels of c-JUN and USP18 in OVCAR-3 cells treated with vehicle control, NaAsO2 (0.5 mM), or NaAsO2(0.5 mM) plus NAC (10 μM) + mito-TEMPO (10 μM). j Cell viability measured by CCK8 assay in OVCAR-3 and A2780 cells overexpressing USP18 and treated with cisplatin (10 μM) plus NAC (10 μM) + mito-TEMPO (10 μM) k RT-qPCR analysis of USP18 mRNA expression levels in OVCAR-3 cells treated with vehicle control, cisplatin (10 μM), or cisplatin combined with c-JUN knockdown. l Schematic of predicted c-JUN binding sites in the USP18 promoter region ( https://jaspar.elixir.no/ ). m ChIP assay in CAOV3 cells validating c-JUN binding to USP18. n-p Dual-luciferase reporter assay confirming c-JUN binding and transcriptional regulation of USP18. n Schematic diagram of the dual-luciferase reporter assay. o Overexpression of c-JUN activates the USP18 promoter. OVCAR-3/CAOV3 cells were co-transfected with the USP18 promoter reporter construct and a c-JUN expression plasmid (or empty vector control). p OVCAR-3 cells transfected with the USP18 promoter reporter were treated with vehicle control, cisplatin (10 μM), or cisplatin (10 μM) combined with c-JUN knockdown (sic-JUN). Data presented as mean ± SD. Significance calculated by unpaired two-tailed t -test (j, k, m, o, p). ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.

    Article Snippet: All sequencing procedures were performed by Annoroad Gene Tech. (Beijing) Co., Ltd.

    Techniques: Expressing, Sequencing, Plasmid Preparation, Control, CCK-8 Assay, Quantitative RT-PCR, Knockdown, Binding Assay, Luciferase, Reporter Assay, Over Expression, Transfection, Construct, Two Tailed Test

    CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of bulk RNA-seq data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.

    Journal: Frontiers in Immunology

    Article Title: CD32b defines distinct dendritic cell lineages generated from the culture of bone marrow with GM-CSF

    doi: 10.3389/fimmu.2026.1703978

    Figure Lengend Snippet: CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of bulk RNA-seq data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.

    Article Snippet: RNA sequencing (RNA-seq) procedure and analysis were performed by Macrogen (Seoul, Korea).

    Techniques: Derivative Assay, RNA Sequencing, Generated, Expressing, Biomarker Discovery, Marker, Fluorescence, Control